rabbit anti trka  (Biosynth Carbosynth)

Journal: Frontiers in Endocrinology

Article Title: Unveiling the TrkA-p35/CDK5 axis: a novel therapeutic target in diabetic kidney disease

doi: 10.3389/fendo.2026.1791283

Figure Lengend Snippet: HG-induced TrkA phosphorylation is associated with podocyte inflammation and injury. (A–D) Representative western blots show the levels of (A) TrkA and phospho-TrkA (Tyr490); (B) ERK, phospho-ERK and EGR1; (C) p35 and CDK5; and (D) IL-1β and TNF-α in MPC5 cells from the LG, HG, and LG + Man groups at 24 h (E) Representative immunofluorescence double staining shows the expression of phospho-TrkA (Tyr490) and synaptopodin in MPC5 cells from the LG, HG, and LG + Man groups. ****p < 0.0001. p < 0.05 was considered statistically significant; ns indicates not significant.

Article Snippet: Anti-phospho-TrkA (pTyr490) antibody (Cat. No. RA18018-100) was purchased from Origene (Rockville, MD, USA).

Techniques: Phospho-proteomics, Western Blot, Immunofluorescence, Double Staining, Expressing

Journal: Frontiers in Endocrinology

Article Title: Unveiling the TrkA-p35/CDK5 axis: a novel therapeutic target in diabetic kidney disease

doi: 10.3389/fendo.2026.1791283

Figure Lengend Snippet: TrkA inhibition reduces the levels of p-TrkA, p-ERK, EGR1, p35, and CDK5 in the kidneys of db/db mice. (A–C) Representative western blots show the protein expression levels of (A) TrkA and phospho-TrkA (Tyr490), (B) ERK, p-ERK and EGR1, and (C) p35 and CDK5 in the kidney samples from the control, db/db, Inh-NC, and Inh-TrkA groups of mice. (D) Representative IF images show the expression of Synaptopodin (red) in the glomeruli of control, db/db, Inh-NC, and Inh-TrkA groups of mice (400X). ***p < 0.001. p < 0.05 was considered statistically significant; ns indicates not significant.

Article Snippet: Anti-phospho-TrkA (pTyr490) antibody (Cat. No. RA18018-100) was purchased from Origene (Rockville, MD, USA).

Techniques: Inhibition, Western Blot, Expressing, Control

Journal: International Journal of Molecular Sciences

Article Title: Evaluation of NTRK Fusions Detection Method in Esophageal Squamous Cell Carcinoma and Gastric Adenocarcinoma

doi: 10.3390/ijms27010336

Figure Lengend Snippet: IHC for TRK, TRKA, TRKB, and TRKC in esophageal squamous cell carcinoma (ESCC). ( A ) Representative images showing immunohistochemical (IHC) staining with anti-pan-TRK antibody in ESCC. Negative TRK staining (left panel), weak positive (middle panel), and moderate positive (right panel) are shown. Scale bars = 250 μm. ( B ) Representative images showing IHC staining using anti-TRKA, anti-TRKB, and anti-TRKC antibodies in ESCC. Positive TRKA, TRKB, and TRKC staining (upper panels) and negative staining (lower panels) are shown. Scale bars = 250 μm.

Article Snippet: After rinsing in PBS, the sections were incubated with ananti-pan-TRK antibody (# EPR17341 ; 1:50 dilution; Abcam, Cambridge, UK), anti-TRKA antibody (#2510; 12G8; 1:400 dilution; Cell Signaling Technology, Danvers, MA, USA), anti-TRKB antibody (#4607; 80G2; 1:2000 dilution; Cell signaling Technology), or anti-TRKC antibody (#3376; C44H5; 1:1000 dilution; Cell Signaling Technology) at 4 °C overnight.

Techniques: Immunohistochemical staining, Immunohistochemistry, Staining, Negative Staining

Journal: bioRxiv

Article Title: Multiple glycoforms of TrkA interact with N-cadherin during trigeminal ganglion neurodevelopment

doi: 10.1101/2025.11.14.688512

Figure Lengend Snippet: (A-C’) Representative transverse sections through the trigeminal ganglion of an E5.5/HH28 chick embryo following immunohistochemistry for N-cadherin (N-cad) and TrkA. Scale bar in (A) is 50 µm and applies to (B, C). Scale bar in (A’) is 50 µm and applies to (B’, C’). (D-I) Representative trigeminal ganglia explant cultures after immunocytochemistry for N-cadherin and TrkA. Scale bar in (D) is 25 µm and applies to (E-I). Arrowheads indicate colocalization of N-cadherin and TrkA. (J, K) Immunoblots of HH28-30 trigeminal ganglia for TrkA with lysate from the embryo ( in vivo ) (J) and from trigeminal ganglia explant cultures ( ex vivo ) (K). Pie charts represent percentage of total signal coming from the different bands of TrkA.

Article Snippet: The membrane was blocked in 5% milk (in 1X PBS+0.1% Tween-20 (PTW)) for 30 minutes at room temperature followed by overnight incubation at 4°C with the following primary antibodies diluted in blocking solution: N-cadherin (MNCD2, DSHB 1:100) and TrkA (MSU, 1:5000 or Origene, 1:1000).

Techniques: Immunohistochemistry, Immunocytochemistry, Western Blot, In Vivo, Ex Vivo

Journal: bioRxiv

Article Title: Multiple glycoforms of TrkA interact with N-cadherin during trigeminal ganglion neurodevelopment

doi: 10.1101/2025.11.14.688512

Figure Lengend Snippet: (A) Dissected trigeminal ganglion from an E5.5/HH28 chick embryo. Dotted lines indicate approximate location where cuts were made to separate trigeminal cell bodies from axons. Scale bar is 1mm. (B-C) N-cadherin-TrkA co-immunoprecipitation on pooled HH28-30 trigeminal cell bodies and axons. Input lanes contain 10% of lysate loaded in immunoprecipitation lanes. OpV, ophthalmic; MmV, maxillomandibular; IP, immunoprecipitation; IgG, Immunoglobulin G. N=3.

Article Snippet: The membrane was blocked in 5% milk (in 1X PBS+0.1% Tween-20 (PTW)) for 30 minutes at room temperature followed by overnight incubation at 4°C with the following primary antibodies diluted in blocking solution: N-cadherin (MNCD2, DSHB 1:100) and TrkA (MSU, 1:5000 or Origene, 1:1000).

Techniques: Immunoprecipitation

Journal: bioRxiv

Article Title: Multiple glycoforms of TrkA interact with N-cadherin during trigeminal ganglion neurodevelopment

doi: 10.1101/2025.11.14.688512

Figure Lengend Snippet: (A) Immunoblots of E6.5/HH28-30 trigeminal ganglia lysate following Mem-PER membrane extraction protocol for TrkA, N-cadherin, Tubb3, GM130 (Golgi protein), and SERCA2 (ER protein) using membrane-extracted and cytosolic-extracted protein fractions. (B) N-cadherin-TrkA co-immunoprecipitation on membrane-extracted fraction. Input contains 10% of lysate loaded in immunoprecipitation lanes. The top blot image is from one experiment using a high exposure and the bottom blot image is from a separate experiment with a lower exposure. IP, immunoprecipitation, IgG, Immunoglobulin G (N=3).

Article Snippet: The membrane was blocked in 5% milk (in 1X PBS+0.1% Tween-20 (PTW)) for 30 minutes at room temperature followed by overnight incubation at 4°C with the following primary antibodies diluted in blocking solution: N-cadherin (MNCD2, DSHB 1:100) and TrkA (MSU, 1:5000 or Origene, 1:1000).

Techniques: Western Blot, Membrane, Extraction, Immunoprecipitation